Liu ZP*, Liu P, Luo D, Liu WX , Wang YR. Exploiting Illumina sequencing for the development of 95 novel polymorphic EST-SSR markers in common vetch (Vicia sativa subsp. sativa). Molecules, 2014, 19: 5777-5789.

影响因子: 2.09

摘要: The common vetch (Vicia sativa subsp. sativa), a self-pollinating and diploidspecies, is one of the most important annual legumes in the world due to its short growthperiod, high nutritional value, and multiple usages as hay, grain, silage, and green manure.The available simple sequence repeat (SSR) markers for common vetch, however, areinsufficient to meet the developing demand for genetic and molecular research on thisimportant species. Here, we aimed to develop and characterise several polymorphicEST-SSR markers from the vetch Illuminatranscriptome. A total number of 1,071potential EST-SSR markers were identified from 1025 unigenes whose lengths weregreater than 1,000 bp, and 450 primer pairs were then designed and synthesized. Finally,95 polymorphic primer pairs were developed for the 10 common vetch accessions, whichincluded 50 individuals. Among the 95 EST-SSR markers, the number of alleles rangedfrom three to 13, and the polymorphism information content values ranged from 0.09 to0.98. The observed heterozygosity values ranged from 0.00 to 1.00, and the expectedheterozygosity values ranged from 0.11 to 0.98. These 95 EST-SSR markers developedfrom the vetch Illuminatranscriptome could greatly promote the development of geneticand molecular breeding studies pertaining to in this species.